RESUMO
A set of 415 clinical samples isolated from 294 patients suspected of having syphilis collected in the Czech Republic between 2004 and 2010 was tested for the presence of treponemal DNA. Standard serological tests showed that 197 patients were syphilis-seropositive and 97 patients were syphilis-seronegative. In each sample, PCR tests for polA (TP0105), tmpC (TP0319), TP0136, TP0548 and 23S rRNA genes were performed. Samples taken from 91 patients were PCR-positive. Molecular typing of treponemal DNA was based on the sequencing of TP0136, TP0548 and 23S rRNA genes. Treponemal DNA was typeable in samples taken from 64 PCR-positive patients and 9 different genotypes were found. The proportion of treponemal strains resistant to macrolide antibiotics was 37.3%. In the DNA samples taken from 39 patients, a parallel treponemal typing approved by Centers for Disease Control and Prevention was performed. The variants of arp and tpr genes appear to combine independently with sequence variants of TP0136, TP0548 and 23S rRNA genes.
Assuntos
DNA Bacteriano/análise , RNA Ribossômico 23S/genética , Ribotipagem , Sífilis/microbiologia , Treponema pallidum/genética , Adulto , Antibacterianos/uso terapêutico , Sequência de Bases , República Tcheca/epidemiologia , Farmacorresistência Bacteriana/genética , Feminino , Variação Genética , Genótipo , Humanos , Masculino , Dados de Sequência Molecular , Fenótipo , Reação em Cadeia da Polimerase , Ribotipagem/métodos , Análise de Sequência de DNA , Sífilis/diagnóstico , Sífilis/tratamento farmacológico , Sífilis/epidemiologia , Treponema pallidum/efeitos dos fármacos , Treponema pallidum/imunologiaAssuntos
Antibacterianos/uso terapêutico , Claritromicina/uso terapêutico , Farmacorresistência Bacteriana , Sífilis/tratamento farmacológico , Treponema pallidum/isolamento & purificação , Sequência de Bases , Cefuroxima/uso terapêutico , DNA Bacteriano/isolamento & purificação , Farmacorresistência Bacteriana/genética , Feminino , Genótipo , Humanos , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Ribotipagem , Sífilis/diagnóstico , Sífilis/microbiologia , Falha de Tratamento , Treponema pallidum/classificação , Treponema pallidum/genética , Adulto JovemRESUMO
We report an occurrence of treatment failure after oral spiramycin therapy in a man with secondary syphilis and a reported penicillin and tetracycline allergy. Molecular detection revealed treponemal DNA in the blood of the patient and sequencing of the 23S rDNA identified an A to G transition at the gene position corresponding to position 2059 in the Escherichia coli 23S rRNA gene. The occurrence of this novel 23S rDNA mutation was examined among 7 rabbit-propagated syphilitic strains of Treponema pallidum and among 22 syphilis patient isolates from the Czech Republic. The prevalence of A2058G and A2059G mutations among clinical specimens was 18.2 and 18.2 %, respectively.
Assuntos
Antibacterianos , Farmacorresistência Bacteriana/genética , Mutação , RNA Ribossômico 23S/genética , Espiramicina , Sífilis/tratamento farmacológico , Treponema pallidum/efeitos dos fármacos , Adolescente , Adulto , Animais , Antibacterianos/farmacologia , Antibacterianos/uso terapêutico , Criança , Pré-Escolar , República Tcheca/epidemiologia , DNA Bacteriano/análise , DNA Bacteriano/isolamento & purificação , Humanos , Lactente , Recém-Nascido , Macrolídeos/farmacologia , Macrolídeos/uso terapêutico , Masculino , Pessoa de Meia-Idade , Prevalência , Coelhos , Análise de Sequência de DNA , Espiramicina/farmacologia , Espiramicina/uso terapêutico , Sífilis/epidemiologia , Sífilis/microbiologia , Falha de Tratamento , Treponema pallidum/genética , Adulto JovemRESUMO
We report here a case of congenital syphilis in a newborn after clindamycin treatment in pregnancy. Using PCR detection of tmpC (TP0319) and DNA sequencing of the genes TP0136 and TP0548, DNA sequences identical to Treponema pallidum subsp. pallidum strain SS14 were detected in the infant's skin lesions, serum, and cerebrospinal fluid.
